Hotspot

trnD-GUC-trnY-GUA

LSC · 29713-30054 · 342 bp

Back to Butia

Pi 0.0146
MarkerSeek score 53.80
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
342
variable_sites
5
pi
0.0146
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GGATCCTAGTTCGGGACTGA AATTCTTCCTGGGTCGATGC 55.0 50.0 57.9 58.0 521 bp 1.0 79.9
trnD-GUC-trnY-GUA_p2 GTGCTCTGACCGATTGAACT AATTCTTCCTGGGTCGATGC 50.0 50.0 57.9 58.0 464 bp 1.0 79.8
trnD-GUC-trnY-GUA_p3 GGATCCTAGTTCGGGACTGA GGGGACGGACTGTAAATTCG 55.0 55.0 57.9 58.4 488 bp 1.0 77.5
trnD-GUC-trnY-GUA_p4 ATCGGATCCTAGTTCGGGAC AATTCTTCCTGGGTCGATGC 55.0 50.0 58.4 58.0 524 bp 1.0 77.4
trnD-GUC-trnY-GUA_p5 CATCGGATCCTAGTTCGGGA AATTCTTCCTGGGTCGATGC 55.0 50.0 58.4 58.0 525 bp 1.0 77.3