Hotspot

ndhF-rpl32

SSC · 116689-117753 · 1065 bp

Back to Bursera

Pi 0.0151

MarkerSeek score 47.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1065
variable_sites: 30
pi: 0.0151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACATAGGAACCGGAAGTGGA	ACGAAAAAGCTTTCAACGCC	50.0	45.0	58.1	58.2	1215-1237 bp	1.0	82.5
ndhF-rpl32_p2	TGTTGCCGTCGGAAAAAGTA	ACGAAAAAGCTTTCAACGCC	45.0	45.0	58.0	58.2	1253-1275 bp	1.0	82.4
ndhF-rpl32_p3	ACACATCCCAACCAATTCCC	ACGAAAAAGCTTTCAACGCC	50.0	45.0	58.1	58.2	1625-1647 bp	1.0	81.5
ndhF-rpl32_p4	CACATCCCAACCAATTCCCA	ACGAAAAAGCTTTCAACGCC	50.0	45.0	58.1	58.2	1624-1646 bp	1.0	81.5
ndhF-rpl32_p5	AGTGGGAATTGTGCGGATTT	ACGAAAAAGCTTTCAACGCC	45.0	45.0	58.1	58.2	1891-1913 bp	1.0	81.0