Hotspot

psaI-pafII

LSC · 62258-62711 · 454 bp

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Pi 0.011

MarkerSeek score 59.30

Primer available yes

Label psaI-pafII

Feature Metadata

label_name: psaI-pafII
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 10
pi: 0.011
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-pafII_p1	ATTTCCGGCAATTGCAATGG	TTGTTTTGCGAGATCCTGCT	45.0	45.0	58.0	58.1	566-575 bp	1.0	81.5
psaI-pafII_p2	ATTTCCGGCAATTGCAATGG	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.0	57.9	590-599 bp	1.0	81.0
psaI-pafII_p3	ATTTCCGGCAATTGCAATGG	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.0	57.9	593-602 bp	1.0	81.0
psaI-pafII_p4	GGCAATTGCAATGGCTTCTT	TTGTTTTGCGAGATCCTGCT	45.0	45.0	57.9	58.1	560-569 bp	1.0	80.9
psaI-pafII_p5	GGCAATTGCAATGGCTTCTT	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	584-593 bp	1.0	80.5