Hotspot

petN-psbM

LSC · 30394-31032 · 639 bp

Back to Bursera

Pi 0.00984

MarkerSeek score 56.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 12
pi: 0.00984
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GCAAAGGTCTTGGGTCAGAA	AGTGGAAAGCGCTTAGATGG	50.0	50.0	58.0	58.0	1287-1317 bp	1.0	83.7
petN-psbM_p2	TTCAGCAAAGGTCTTGGGTC	AGTGGAAAGCGCTTAGATGG	50.0	50.0	58.0	58.0	1291-1321 bp	1.0	83.7
petN-psbM_p3	TGAAAGTCTCTGAGTGCACG	AGTGGAAAGCGCTTAGATGG	50.0	50.0	57.9	58.0	1532-1563 bp	1.0	82.3
petN-psbM_p4	GCAAAGGTCTTGGGTCAGAA	GGAAGTGGAAAGCGCTTAGA	50.0	50.0	58.0	57.9	1290-1320 bp	0.8	74.4
petN-psbM_p5	TTCAGCAAAGGTCTTGGGTC	GGAAGTGGAAAGCGCTTAGA	50.0	50.0	58.0	57.9	1294-1324 bp	0.8	74.3