Hotspot

trnR-UCU-atpA

LSC · 11576-11740 · 165 bp

Back to Bursera

Pi 0.0315

MarkerSeek score 57.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 10
pi: 0.0315
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	CGAGGAAGCAGAAACCCTTT	50.0	50.0	58.0	58.1	520-527 bp	1.0	82.5
trnR-UCU-atpA_p2	AGCCTTCCAAGCTAACGATG	CATTCACCGAGGAAGCAGAA	50.0	50.0	58.0	57.9	527-534 bp	1.0	82.4
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	AAGACATTCACCGAGGAAGC	50.0	50.0	58.0	57.9	531-538 bp	1.0	82.4
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	ATGAAACAAGTAGCCGGCAA	50.0	45.0	58.0	58.1	843-850 bp	1.0	81.4
trnR-UCU-atpA_p5	AGCCTTCCAAGCTAACGATG	TGGAAAGAGCCGCGAAATTA	50.0	45.0	58.0	57.9	1073-1080 bp	1.0	80.9