Hotspot

trnS-GCU-trnG-UCC

LSC · 9603-10550 · 948 bp

Back to Bursera

Pi 0.012

MarkerSeek score 66.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 948
variable_sites: 22
pi: 0.012
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	AACGGAAGGACCCCTTAACT	50.0	50.0	58.0	58.3	1045-1065 bp	1.0	81.8
trnS-GCU-trnG-UCC_p2	GATACCAAGCCAGCAATGGA	AACGGAAGGACCCCTTAACT	50.0	50.0	57.9	58.3	1124-1144 bp	1.0	81.1
trnS-GCU-trnG-UCC_p3	AGATACCAAGCCAGCAATGG	AACGGAAGGACCCCTTAACT	50.0	50.0	57.9	58.3	1125-1145 bp	1.0	81.1
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	AACGGAAGGACCCCTTAACT	50.0	50.0	58.2	58.3	1103-1123 bp	1.0	80.4
trnS-GCU-trnG-UCC_p5	ACGCTTTAATCCACTCAGCC	AACGGAAGGACCCCTTAACT	50.0	50.0	58.3	58.3	1042-1062 bp	1.0	79.8