Hotspot

rps16-trnQ-UUG

LSC · 6648-8242 · 1595 bp

Back to Bursera

Pi 0.0179
MarkerSeek score 64.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1595
variable_sites
56
pi
0.0179
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1726-1766 bp 1.0 81.8
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1668-1708 bp 1.0 81.4
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAAATTGAAATGGGGCGTGG 50.0 50.0 58.2 58.0 1737-1777 bp 1.0 81.3
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1679-1719 bp 1.0 80.8
rps16-trnQ-UUG_p5 CCACAGCTGATCATGTCCTT GAAATTGAAATGGGGCGTGG 50.0 50.0 57.6 58.0 1747-1787 bp 1.0 79.6