Hotspot

trnK-UUU-rps16

LSC · 4456-5501 · 1046 bp

Back to Bursera

Pi 0.0113

MarkerSeek score 53.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 22
pi: 0.0113
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	GCCGTACGAGGAGAAAACTT	55.0	50.0	58.0	57.9	1459-1486 bp	1.0	81.6
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	CAAAAAGGGGTGTGTTGCTG	55.0	50.0	58.0	58.1	1866-1901 bp	1.0	81.5
trnK-UUU-rps16_p3	TCAGGATCAGTCGTGGTCTT	CAAAAAGGGGTGTGTTGCTG	50.0	50.0	58.1	58.1	1862-1897 bp	1.0	81.0
trnK-UUU-rps16_p4	TTCAGGATCAGTCGTGGTCT	CAAAAAGGGGTGTGTTGCTG	50.0	50.0	58.1	58.1	1863-1898 bp	1.0	81.0
trnK-UUU-rps16_p5	CCGATGGTATGGACGAATCC	CAAAAAGGGGTGTGTTGCTG	55.0	50.0	57.9	58.1	1832-1867 bp	1.0	81.0