Hotspot

ndhD-ccsA

SSC · 123374-123614 · 241 bp

Back to Burmeistera

Pi 0.0205

MarkerSeek score 62.40

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 241
variable_sites: 22
pi: 0.0205
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	AGGGATTGGAATCTACCCGG	TCTGGGGTTTCTGATAATTTGGA	55.0	39.1	58.6	57.5	412-444 bp	1.0	53.0
ndhD-ccsA_p2	GGGATTGGAATCTACCCGGA	TCTGGGGTTTCTGATAATTTGGA	55.0	39.1	58.6	57.5	411-443 bp	1.0	53.0
ndhD-ccsA_p3	AGGGATTGGAATCTACCCGG	AGGGCTACATAGTTATGGTTCA	55.0	40.9	58.6	56.3	358-390 bp	1.0	52.7
ndhD-ccsA_p4	GGGATTGGAATCTACCCGGA	AGGGCTACATAGTTATGGTTCA	55.0	40.9	58.6	56.3	357-389 bp	1.0	52.7
ndhD-ccsA_p5	ACCCGGATTTTGTTCTGTCA	TCTGGGGTTTCTGATAATTTGGA	45.0	39.1	57.4	57.5	398-430 bp	1.0	52.5