Hotspot

petG-trnW (CCA)

LSC · 65616-65716 · 101 bp

Back to Burmeistera

Pi 0.0266

MarkerSeek score 60.20

Primer available yes

Label petG-trnW (CCA)

Feature Metadata

label_name: petG-trnW (CCA)
feature_type: igs
region: LSC
length_bp: 101
variable_sites: 10
pi: 0.0266
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW (CCA)_p1	GCGATCAGTTGGACCTTTGA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.2	58.0	174-175 bp	1.0	82.8
petG-trnW (CCA)_p2	GCGATCAGTTGGACCTTTGA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.2	57.9	160-161 bp	1.0	82.8
petG-trnW (CCA)_p3	GCGATCAGTTGGACCTTTGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.2	58.0	196-197 bp	1.0	82.4
petG-trnW (CCA)_p4	GCGATCAGTTGGACCTTTGA	TTCAAATCCTACAGAGCGCG	50.0	50.0	58.2	58.4	141-142 bp	1.0	81.3
petG-trnW (CCA)_p5	GCGATCAGTTGGACCTTTGA	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	58.2	57.7	194-195 bp	1.0	79.8