Hotspot

psbE-petL

LSC · 63928-65228 · 1301 bp

Back to Burmeistera

Pi 0.00867

MarkerSeek score 60.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1301
variable_sites: 54
pi: 0.00867
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1329-1378 bp	0.941176	83.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1331-1380 bp	0.941176	83.3
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.2	58.0	1415-1464 bp	0.941176	83.1
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.2	58.0	1417-1466 bp	0.941176	83.0
psbE-petL_p5	TGCTGACGAATAACCAACCC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.2	58.0	1420-1469 bp	0.941176	83.0