Hotspot

ndhC-psaI

LSC · 57293-58333 · 1041 bp

Back to Burmeistera

Pi 0.0213

MarkerSeek score 64.70

Primer available yes

Label ndhC-psaI

Feature Metadata

label_name: ndhC-psaI
feature_type: igs
region: LSC
length_bp: 1041
variable_sites: 95
pi: 0.0213
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-psaI_p1	TGCTAATGGGGGCTAAGACT	AAGACCTGCGATCCCATAGA	50.0	50.0	58.1	57.9	1821-2112 bp	0.941176	81.1
ndhC-psaI_p2	TGCTAATGGGGGCTAAGACT	AGACCTGCGATCCCATAGAA	50.0	50.0	58.1	57.9	1820-2111 bp	0.941176	81.1
ndhC-psaI_p3	TTGAGCGTGGTACTAGTCCA	AAGACCTGCGATCCCATAGA	50.0	50.0	58.1	57.9	2330-2621 bp	0.941176	80.3
ndhC-psaI_p4	TTGAGCGTGGTACTAGTCCA	AGACCTGCGATCCCATAGAA	50.0	50.0	58.1	57.9	2329-2620 bp	0.941176	80.3
ndhC-psaI_p5	TGAGCGTGGTACTAGTCCAT	AGACCTGCGATCCCATAGAA	50.0	50.0	57.9	57.9	2328-2619 bp	0.941176	79.9