Hotspot

trnT (UGU)-trnL (UAA)

LSC · 53318-53994 · 677 bp

Back to Burmeistera

Pi 0.0144

MarkerSeek score 73.70

Primer available yes

Label trnT (UGU)-trnL (UAA)

Feature Metadata

label_name: trnT (UGU)-trnL (UAA)
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 47
pi: 0.0144
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT (UGU)-trnL (UAA)_p1	GCGATGCTCTACCTTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	604-749 bp	1.0	88.1
trnT (UGU)-trnL (UAA)_p2	GCGATGCTCTACCTTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	612-757 bp	1.0	87.6
trnT (UGU)-trnL (UAA)_p3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	636-781 bp	1.0	87.0
trnT (UGU)-trnL (UAA)_p4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	644-789 bp	1.0	86.6
trnT (UGU)-trnL (UAA)_p5	GCGATGCTCTACCTTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	618-763 bp	1.0	86.2