Hotspot

trnS (GCU)-trnG (UCC)

LSC · 14648-15367 · 720 bp

Back to Burmeistera

Pi 0.0118
MarkerSeek score 64.10
Primer available yes
Label trnS (GCU)-trnG (UCC)

Feature Metadata

label_name
trnS (GCU)-trnG (UCC)
feature_type
igs
region
LSC
length_bp
720
variable_sites
44
pi
0.0118
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS (GCU)-trnG (UCC)_p1 ACGGAAAGAGAGGGATTCGA AGGAGGTTCATAGAACGAATCAC 50.0 43.5 58.2 58.0 738-873 bp 0.941176 60.6
trnS (GCU)-trnG (UCC)_p2 ACGAAAACGGAAAGAGAGGG AGGAGGTTCATAGAACGAATCAC 50.0 43.5 57.8 58.0 744-879 bp 0.941176 60.5
trnS (GCU)-trnG (UCC)_p3 ACGGAAAGAGAGGGATTCGA GGAGGTTCATAGAACGAATCACA 50.0 43.5 58.2 58.2 737-872 bp 0.941176 58.7
trnS (GCU)-trnG (UCC)_p4 ACGAAAACGGAAAGAGAGGG GGAGGTTCATAGAACGAATCACA 50.0 43.5 57.8 58.2 743-878 bp 0.941176 58.6
trnS (GCU)-trnG (UCC)_p5 AGTCATCGACGAAAACGGAA AGGAGGTTCATAGAACGAATCAC 45.0 43.5 57.6 58.0 752-887 bp 0.941176 58.4