Hotspot

trnH (GUG)-psbA

LSC · 78-411 · 334 bp

Back to Burmeistera

Pi 0.0128
MarkerSeek score 60.40
Primer available yes
Label trnH (GUG)-psbA

Feature Metadata

label_name
trnH (GUG)-psbA
feature_type
igs
region
LSC
length_bp
334
variable_sites
23
pi
0.0128
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH (GUG)-psbA_p1 CAATCCACTGCCTTCATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 466-477 bp 1.0 81.0
trnH (GUG)-psbA_p2 AATCCACTGCCTTCATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 465-476 bp 1.0 81.0
trnH (GUG)-psbA_p3 ACAATCCACTGCCTTCATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 467-478 bp 1.0 81.0
trnH (GUG)-psbA_p4 CAATCCACTGCCTTCATCCA ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 467-478 bp 1.0 80.5
trnH (GUG)-psbA_p5 AATCCACTGCCTTCATCCAC ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 466-477 bp 1.0 80.5