primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH (GUG)-psbA_p1	trnH (GUG)-psbA	1	CAATCCACTGCCTTCATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	78	411	466-477	1.000000	81.0
trnH (GUG)-psbA_p2	trnH (GUG)-psbA	2	AATCCACTGCCTTCATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	411	465-476	1.000000	81.0
trnH (GUG)-psbA_p3	trnH (GUG)-psbA	3	ACAATCCACTGCCTTCATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	411	467-478	1.000000	81.0
trnH (GUG)-psbA_p4	trnH (GUG)-psbA	4	CAATCCACTGCCTTCATCCA	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.529871	78	411	467-478	1.000000	80.5
trnH (GUG)-psbA_p5	trnH (GUG)-psbA	5	AATCCACTGCCTTCATCCAC	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.531379	78	411	466-477	1.000000	80.5
trnK (UUU)-rps16_p1	trnK (UUU)-rps16	1	TAAGAATGGGAGCTCGTTGC	GCCCAAAAACCAAAAACCGA	20	20	50.0	45.0	58.0	58.0	7.8	0.0	0.049764	10757	11336	1387-1452	1.000000	90.8
trnK (UUU)-rps16_p2	trnK (UUU)-rps16	2	TAAGAATGGGAGCTCGTTGC	CTCAATCCTCGGAACCTTGG	20	20	50.0	55.0	58.0	58.0	7.8	0.0	0.054014	10757	11336	1366-1431	1.000000	90.8
trnK (UUU)-rps16_p3	trnK (UUU)-rps16	3	TAAGAATGGGAGCTCGTTGC	CGTTGCAATTGATGTTCGCT	20	20	50.0	45.0	58.0	58.0	7.8	10.3	0.056414	10757	11336	1094-1135	0.941176	85.3
trnK (UUU)-rps16_p4	trnK (UUU)-rps16	4	TAAGAATGGGAGCTCGTTGC	CGCACTCTTCCAATAAGGCT	20	20	50.0	50.0	58.0	58.0	7.8	0.0	0.054014	10757	11336	1569-1628	0.470588	64.5
trnK (UUU)-rps16_p5	trnK (UUU)-rps16	5	TAAGAATGGGAGCTCGTTGC	GAAAGGTGGCTCACTTCCAA	20	20	50.0	50.0	58.0	58.0	7.8	5.5	0.042919	10757	11336	1477-1525	0.294118	56.5
rps16-trnQ (UUG)_p1	rps16-trnQ (UUG)	1	AGCGAACATCAATTGCAACG	CCAAGATGACTTCATGCCGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.060658	11604	12918	1953-2637	1.000000	91.5
rps16-trnQ (UUG)_p2	rps16-trnQ (UUG)	2	GCGAACATCAATTGCAACGA	CCAAGATGACTTCATGCCGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.066008	11604	12918	1952-2636	1.000000	91.5
rps16-trnQ (UUG)_p3	rps16-trnQ (UUG)	3	TTGCTTTCTACCACAGCGTT	CCAAGATGACTTCATGCCGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.066626	11604	12918	1916-2600	1.000000	91.5
rps16-trnQ (UUG)_p4	rps16-trnQ (UUG)	4	AGCGAACATCAATTGCAACG	CTTACAGCAGCTTGCCAAAC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.088333	11604	12918	1674-2358	1.000000	91.3
rps16-trnQ (UUG)_p5	rps16-trnQ (UUG)	5	TGCTTTCTACCACAGCGTTT	CCAAGATGACTTCATGCCGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.066626	11604	12918	1915-2599	0.882353	86.8
trnQ (UUG)-psbK_p1	trnQ (UUG)-psbK	1	TTGGAAGTGAGCCACCTTTC	CCAGGTCCGTGGAAAGAAAA	20	20	50.0	50.0	58.0	58.0	2.2	0.0	0.043084	12991	13709	2290-2977	0.882353	86.9
trnQ (UUG)-psbK_p2	trnQ (UUG)-psbK	2	TTGGAAGTGAGCCACCTTTC	CCAAGATGACTTCATGCCGA	20	20	50.0	50.0	58.0	58.0	2.2	0.0	0.047163	12991	13709	1566-2253	0.882353	86.9
trnQ (UUG)-psbK_p3	trnQ (UUG)-psbK	3	TGCTTGTAGTCTTTCGGGTG	CCAAGATGACTTCATGCCGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.192238	12991	13709	1340-1824	0.823529	83.4
trnQ (UUG)-psbK_p4	trnQ (UUG)-psbK	4	CGGCAATACCCATAGCACAT	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.159875	12991	13709	784-1153	0.411765	63.9
trnQ (UUG)-psbK_p5	trnQ (UUG)-psbK	5	CGGCAATACCCATAGCACAT	CCAAGATGACTTCATGCCGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.132200	12991	13709	1063-1432	0.411765	63.7
trnS (GCU)-trnG (UCC)_p1	trnS (GCU)-trnG (UCC)	1	ACGGAAAGAGAGGGATTCGA	AGGAGGTTCATAGAACGAATCAC	20	23	50.0	43.5	58.2	58.0	0.0	4.0	3.163677	14648	15367	738-873	0.941176	60.6
trnS (GCU)-trnG (UCC)_p2	trnS (GCU)-trnG (UCC)	2	ACGAAAACGGAAAGAGAGGG	AGGAGGTTCATAGAACGAATCAC	20	23	50.0	43.5	57.8	58.0	0.0	4.0	3.177647	14648	15367	744-879	0.941176	60.5
trnS (GCU)-trnG (UCC)_p3	trnS (GCU)-trnG (UCC)	3	ACGGAAAGAGAGGGATTCGA	GGAGGTTCATAGAACGAATCACA	20	23	50.0	43.5	58.2	58.2	0.0	4.0	3.395937	14648	15367	737-872	0.941176	58.7
trnS (GCU)-trnG (UCC)_p4	trnS (GCU)-trnG (UCC)	4	ACGAAAACGGAAAGAGAGGG	GGAGGTTCATAGAACGAATCACA	20	23	50.0	43.5	57.8	58.2	0.0	4.0	3.409907	14648	15367	743-878	0.941176	58.6
trnS (GCU)-trnG (UCC)_p5	trnS (GCU)-trnG (UCC)	5	AGTCATCGACGAAAACGGAA	AGGAGGTTCATAGAACGAATCAC	20	23	45.0	43.5	57.6	58.0	0.0	4.0	3.439560	14648	15367	752-887	0.941176	58.4
rpoB-trnC (GCA)_p1	rpoB-trnC (GCA)	1	CCTGATCGATGAACCTGCAA	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.5	0.0	0.047163	33131	35078	2299-2406	1.000000	85.8
rpoB-trnC (GCA)_p2	rpoB-trnC (GCA)	2	AGTCCCGCGGATACATAGAA	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	30.7	0.0	0.027622	33131	35078	2468-2575	1.000000	85.7
rpoB-trnC (GCA)_p3	rpoB-trnC (GCA)	3	TAGACTCGACCCCATAGCTG	GGTGCAGAGTCAAAAAGGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.036083	33131	35078	2784-2891	1.000000	85.3
rpoB-trnC (GCA)_p4	rpoB-trnC (GCA)	4	TCGACCCCATAGCTGATGAT	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034200	33131	35078	2779-2886	0.941176	82.7
rpoB-trnC (GCA)_p5	rpoB-trnC (GCA)	5	ATTGGATCCCCACCTACACA	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	9.9	0.0	0.038934	33131	35078	2924-2993	0.764706	74.8
trnT (UGU)-trnL (UAA)_p1	trnT (UGU)-trnL (UAA)	1	GCGATGCTCTACCTTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	53318	53994	604-749	1.000000	88.1
trnT (UGU)-trnL (UAA)_p2	trnT (UGU)-trnL (UAA)	2	GCGATGCTCTACCTTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	53318	53994	612-757	1.000000	87.6
trnT (UGU)-trnL (UAA)_p3	trnT (UGU)-trnL (UAA)	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	53318	53994	636-781	1.000000	87.0
trnT (UGU)-trnL (UAA)_p4	trnT (UGU)-trnL (UAA)	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	53318	53994	644-789	1.000000	86.6
trnT (UGU)-trnL (UAA)_p5	trnT (UGU)-trnL (UAA)	5	GCGATGCTCTACCTTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	53318	53994	618-763	1.000000	86.2
ndhC-psaI_p1	ndhC-psaI	1	TGCTAATGGGGGCTAAGACT	AAGACCTGCGATCCCATAGA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.194789	57293	58333	1821-2112	0.941176	81.1
ndhC-psaI_p2	ndhC-psaI	2	TGCTAATGGGGGCTAAGACT	AGACCTGCGATCCCATAGAA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.194789	57293	58333	1820-2111	0.941176	81.1
ndhC-psaI_p3	ndhC-psaI	3	TTGAGCGTGGTACTAGTCCA	AAGACCTGCGATCCCATAGA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.174326	57293	58333	2330-2621	0.941176	80.3
ndhC-psaI_p4	ndhC-psaI	4	TTGAGCGTGGTACTAGTCCA	AGACCTGCGATCCCATAGAA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.174326	57293	58333	2329-2620	0.941176	80.3
ndhC-psaI_p5	ndhC-psaI	5	TGAGCGTGGTACTAGTCCAT	AGACCTGCGATCCCATAGAA	20	20	50.0	50.0	57.9	57.9	14.6	0.0	0.227428	57293	58333	2328-2619	0.941176	79.9
petA-psbJ_p1	petA-psbJ	1	AGCAGTTCGAGAAGGTTCAA	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.1	57.6	0.0	4.1	1.275660	62168	63140	985-1047	1.000000	78.9
petA-psbJ_p2	petA-psbJ	2	AAGCAGTTCGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.1	57.6	0.0	4.1	1.275660	62168	63140	986-1048	1.000000	78.8
petA-psbJ_p3	petA-psbJ	3	AGAAGCAGTTCGAGAAGGTTC	ATTCCGGATTGGGTTCATCC	21	20	47.6	50.0	58.0	57.6	13.6	4.1	1.398340	62168	63140	988-1050	1.000000	77.9
petA-psbJ_p4	petA-psbJ	4	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	56.8	57.6	5.1	4.1	1.527465	62168	63140	978-1040	1.000000	76.9
petA-psbJ_p5	petA-psbJ	5	AAGCAGTTCGAGAAGGTTCAA	ATTCCGGATTGGGTTCATCC	21	20	42.9	50.0	57.8	57.6	0.0	4.1	1.558977	62168	63140	986-1048	1.000000	76.6
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.156772	63928	65228	1329-1378	0.941176	83.3
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.162314	63928	65228	1331-1380	0.941176	83.3
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.171450	63928	65228	1415-1464	0.941176	83.1
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	AAGCCGCTAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.176991	63928	65228	1417-1466	0.941176	83.0
psbE-petL_p5	psbE-petL	5	TGCTGACGAATAACCAACCC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.184355	63928	65228	1420-1469	0.941176	83.0
petG-trnW (CCA)_p1	petG-trnW (CCA)	1	GCGATCAGTTGGACCTTTGA	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.228417	65616	65716	174-175	1.000000	82.8
petG-trnW (CCA)_p2	petG-trnW (CCA)	2	GCGATCAGTTGGACCTTTGA	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	58.2	57.9	0.0	1.4	0.280539	65616	65716	160-161	1.000000	82.8
petG-trnW (CCA)_p3	petG-trnW (CCA)	3	GCGATCAGTTGGACCTTTGA	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.2	58.0	0.0	2.6	0.207617	65616	65716	196-197	1.000000	82.4
petG-trnW (CCA)_p4	petG-trnW (CCA)	4	GCGATCAGTTGGACCTTTGA	TTCAAATCCTACAGAGCGCG	20	20	50.0	50.0	58.2	58.4	0.0	0.0	0.551162	65616	65716	141-142	1.000000	81.3
petG-trnW (CCA)_p5	petG-trnW (CCA)	5	GCGATCAGTTGGACCTTTGA	CGCTCTTAGTTCAGTTCGGT	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.540886	65616	65716	194-195	1.000000	79.8
ycf1_p1	ycf1	1	CCCAGAGCGAATTCGAATGA	CGAAATCTACTAGCCTCGGC	20	20	50.0	55.0	58.1	57.9	31.0	0.0	0.113308	114020	114139	442-661	1.000000	87.6
ycf1_p2	ycf1	2	CCCAGAGCGAATTCGAATGA	CATCAAACCAAATTCCGCCC	20	20	50.0	50.0	58.1	58.0	31.0	0.0	0.068777	114020	114139	675-894	1.000000	87.1
ycf1_p3	ycf1	3	CCCAGAGCGAATTCGAATGA	CGCCCAGCTAAACAGAGTAG	20	20	50.0	55.0	58.1	58.1	31.0	0.0	0.132131	114020	114139	660-879	1.000000	86.7
ycf1_p4	ycf1	4	CCCAGAGCGAATTCGAATGA	CGGCAGATCTCCCATTTTCA	20	20	50.0	50.0	58.1	58.0	31.0	2.9	0.102712	114020	114139	606-825	0.941176	84.1
ycf1_p5	ycf1	5	CCCAGAGCGAATTCGAATGA	CCCACCATCTGCATTAAGCT	20	20	50.0	50.0	58.1	57.9	31.0	0.0	0.122996	114020	114139	626-758	0.882353	82.3
ndhD-ccsA_p1	ndhD-ccsA	1	AGGGATTGGAATCTACCCGG	TCTGGGGTTTCTGATAATTTGGA	20	23	55.0	39.1	58.6	57.5	0.0	0.0	4.093344	123374	123614	412-444	1.000000	53.0
ndhD-ccsA_p2	ndhD-ccsA	2	GGGATTGGAATCTACCCGGA	TCTGGGGTTTCTGATAATTTGGA	20	23	55.0	39.1	58.6	57.5	0.7	0.0	4.097608	123374	123614	411-443	1.000000	53.0
ndhD-ccsA_p3	ndhD-ccsA	3	AGGGATTGGAATCTACCCGG	AGGGCTACATAGTTATGGTTCA	20	22	55.0	40.9	58.6	56.3	0.0	4.3	4.239958	123374	123614	358-390	1.000000	52.7
ndhD-ccsA_p4	ndhD-ccsA	4	GGGATTGGAATCTACCCGGA	AGGGCTACATAGTTATGGTTCA	20	22	55.0	40.9	58.6	56.3	0.7	4.3	4.244222	123374	123614	357-389	1.000000	52.7
ndhD-ccsA_p5	ndhD-ccsA	5	ACCCGGATTTTGTTCTGTCA	TCTGGGGTTTCTGATAATTTGGA	20	23	45.0	39.1	57.4	57.5	0.7	0.0	4.178131	123374	123614	398-430	1.000000	52.5
trnL (UAG)-rpl32_p1	trnL (UAG)-rpl32	1	AGCGTGTCTACCGATTTCAC	GGAAGGGATATTCATCGGCG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.280977	124737	125200	580-592	1.000000	85.0
trnL (UAG)-rpl32_p2	trnL (UAG)-rpl32	2	CAGCGTGTCTACCGATTTCA	GGAAGGGATATTCATCGGCG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.282347	124737	125200	581-593	1.000000	85.0
trnL (UAG)-rpl32_p3	trnL (UAG)-rpl32	3	AGCGTGTCTACCGATTTCAC	ACACTGAGTCACCTCTTCGT	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.314204	124737	125200	670-682	1.000000	83.8
trnL (UAG)-rpl32_p4	trnL (UAG)-rpl32	4	CAGCGTGTCTACCGATTTCA	ACACTGAGTCACCTCTTCGT	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.315574	124737	125200	671-683	1.000000	83.7
trnL (UAG)-rpl32_p5	trnL (UAG)-rpl32	5	GGTGTTTTGAAGACGGCATG	GGAAGGGATATTCATCGGCG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.323624	124737	125200	649-661	0.941176	81.7
rpl32-ndhF_p1	rpl32-ndhF	1	CGCCGATGAATATCCCTTCC	AGGAACTGAAAGGGGAAGGA	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.407005	125363	126174	880-996	1.000000	85.2
rpl32-ndhF_p2	rpl32-ndhF	2	CGCCGATGAATATCCCTTCC	GGAACTGAAAGGGGAAGGAA	20	20	55.0	50.0	58.3	57.0	0.0	0.0	1.245047	125363	126174	879-995	1.000000	78.5
rpl32-ndhF_p3	rpl32-ndhF	3	CGCCGATGAATATCCCTTCC	ACTGAAAGGGGAAGGAAAGG	20	20	55.0	50.0	58.3	57.0	0.0	0.0	1.253393	125363	126174	876-992	1.000000	78.4
rpl32-ndhF_p4	rpl32-ndhF	4	CGCCGATGAATATCCCTTCC	CTGAAAGGGGAAGGAAAGGT	20	20	55.0	50.0	58.3	57.0	0.0	0.0	1.253393	125363	126174	875-991	1.000000	78.4
rpl32-ndhF_p5	rpl32-ndhF	5	GCCGATGAATATCCCTTCCT	AGGAACTGAAAGGGGAAGGA	20	20	50.0	50.0	56.8	57.9	0.0	0.0	1.294709	125363	126174	879-995	1.000000	78.1