Hotspot

rpl16

LSC · 80448-81946 · 1499 bp

Back to Burmannia

Pi 0.0627

MarkerSeek score 82.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1499
variable_sites: 133
pi: 0.0627
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TAGGCACTGCGTCTTTGATC	ATACAAATTGCGGGGCGTAT	50.0	45.0	58.0	58.0	2122-2186 bp	0.666667	73.7
rpl16_p2	GCGTCTTTGATCACAGCAAC	ATACAAATTGCGGGGCGTAT	50.0	45.0	58.0	58.0	2114-2178 bp	0.666667	73.5
rpl16_p3	AATTCCCGAGTAATCGCACC	ATACAAATTGCGGGGCGTAT	50.0	45.0	58.1	58.0	2294-2358 bp	0.666667	73.0
rpl16_p4	TAGGCACTGCGTCTTTGATC	GTCGAACACGAGGAATCGAA	50.0	50.0	58.0	58.0	2297-2346 bp	0.666667	72.8
rpl16_p5	GCGTCTTTGATCACAGCAAC	GTCGAACACGAGGAATCGAA	50.0	50.0	58.0	58.0	2289-2338 bp	0.666667	72.5