Hotspot

clpP

LSC · 68729-70874 · 2146 bp

Back to Burmannia

Pi 0.0743

MarkerSeek score 81.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2146
variable_sites: 220
pi: 0.0743
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CATCGAGGCGATTGGGTTTA	CCAATACGCAATGGGAGGAA	50.0	50.0	58.1	57.9	2766-2786 bp	0.666667	73.6
clpP_p2	AGTCGCACATACACCCTAGT	CCAATACGCAATGGGAGGAA	50.0	50.0	58.2	57.9	2587-2603 bp	0.666667	73.0
clpP_p3	GATCTCACTCACATCGAGGC	CCAATACGCAATGGGAGGAA	55.0	50.0	57.9	57.9	2777-2797 bp	0.666667	72.9
clpP_p4	ATCGAGGCGATTGGGTTTAC	CCAATACGCAATGGGAGGAA	50.0	50.0	58.1	57.9	2785 bp	0.333333	52.5
clpP_p5	ACCATTTGCTACCAATGGGG	CCAATACGCAATGGGAGGAA	50.0	50.0	58.1	57.9	2827 bp	0.333333	51.8