Hotspot

atpF

LSC · 9723-11101 · 1379 bp

Back to Burmannia

Pi 0.0716

MarkerSeek score 83.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1379
variable_sites: 137
pi: 0.0716
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CCATTAGTACGACGCCAACA	TATTGCGAGACAACCCGAAG	50.0	50.0	58.0	58.0	2066-2269 bp	1.0	92.0
atpF_p2	CCATTAGTACGACGCCAACA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	2167-2370 bp	1.0	91.7
atpF_p3	TTACCCGTCTGTCTATCCCC	TATTGCGAGACAACCCGAAG	55.0	50.0	57.9	58.0	2361-2564 bp	1.0	91.3
atpF_p4	CACGACCCAAATAAGCCTCA	TATTGCGAGACAACCCGAAG	50.0	50.0	57.9	58.0	2156-2359 bp	1.0	91.1
atpF_p5	CCATTAGTACGACGCCAACA	ATCCATTGATTTCTGCCGCT	50.0	45.0	58.0	57.9	2175-2378 bp	0.666667	73.7