Hotspot

rps15-ycf1

SSC · 123772-124187 · 416 bp

Back to Burmannia

Pi 0.179

MarkerSeek score 78.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 416
variable_sites: 96
pi: 0.179
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AAGTCGTTGACGTTTTCCCA	TCAAGCTGTTTCTTTGGCCA	45.0	45.0	58.0	58.2	690-739 bp	0.666667	74.8
rps15-ycf1_p2	TGCATTGATGGATGGTGAGG	TCAAGCTGTTTCTTTGGCCA	50.0	45.0	57.9	58.2	966-1020 bp	0.666667	73.7
rps15-ycf1_p3	CTTCGCCGTCAAGAGTAACA	TCAAGCTGTTTCTTTGGCCA	50.0	45.0	57.9	58.2	1004-1058 bp	0.666667	73.5
rps15-ycf1_p4	AAGTCGTTGACGTTTTCCCA	TGTTGTTGGCGAGAGGAATT	45.0	45.0	58.0	57.7	1014 bp	0.333333	51.0
rps15-ycf1_p5	AAGTCGTTGACGTTTTCCCA	GCGAGAGGAATTCGGATAGC	45.0	55.0	58.0	58.3	1006 bp	0.333333	50.7