Hotspot

rbcL-accD

LSC · 54823-55753 · 931 bp

Back to Burmannia

Pi 0.11

MarkerSeek score 82.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 931
variable_sites: 133
pi: 0.11
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ATTGGGTCTCTATGGCAGGT	TTCATGGTCTTTGCCCTCAC	50.0	50.0	58.1	58.1	1336-1389 bp	1.0	90.5
rbcL-accD_p2	CCTGGTGCAGTAGCTAATCG	TTCATGGTCTTTGCCCTCAC	55.0	50.0	58.1	58.1	1194-1246 bp	1.0	90.1
rbcL-accD_p3	TTCCGTCCTCCAATTTGGTG	TTCATGGTCTTTGCCCTCAC	50.0	50.0	58.1	58.1	1266-1298 bp	0.666667	75.1
rbcL-accD_p4	GGGCTGTATTTGCGAGAGAA	TTCATGGTCTTTGCCCTCAC	50.0	50.0	58.0	58.1	1699-1731 bp	0.666667	74.0
rbcL-accD_p5	AAGGGCTGTATTTGCGAGAG	TTCATGGTCTTTGCCCTCAC	50.0	50.0	58.0	58.1	1701-1733 bp	0.666667	73.9