Hotspot

trnT-UGU-trnL-UAA

LSC · 44970-45596 · 627 bp

Back to Burmannia

Pi 0.12

MarkerSeek score 82.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 627
variable_sites: 101
pi: 0.12
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CGAAGCCATACCCAATCGAA	TTTGTTTGCAGCCCATTTGG	50.0	45.0	58.1	58.0	2073 bp	0.333333	52.6
trnT-UGU-trnL-UAA_p2	CGAAGCCATACCCAATCGAA	CAGCCCATTTGGAATCGGTA	50.0	50.0	58.1	57.9	2065 bp	0.333333	52.5
trnT-UGU-trnL-UAA_p3	AAAACCTGACCCGTTGATCC	TTTGTTTGCAGCCCATTTGG	50.0	45.0	58.1	58.0	2015 bp	0.333333	52.3
trnT-UGU-trnL-UAA_p4	AAAACCTGACCCGTTGATCC	CAGCCCATTTGGAATCGGTA	50.0	50.0	58.1	57.9	2007 bp	0.333333	52.2
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	TTTGTTTGCAGCCCATTTGG	55.0	45.0	57.9	58.0	1407 bp	0.333333	52.0