Hotspot

ycf3-trnS-GGA

LSC · 42817-43509 · 693 bp

Back to Burmannia

Pi 0.107

MarkerSeek score 81.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 97
pi: 0.107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTTTCCCCTGAAGTTGTCGG	ATCCCCACATCAGGAATTGC	50.0	50.0	58.0	57.9	1320-1400 bp	0.666667	74.7
ycf3-trnS-GGA_p2	TTTTCCCCTGAAGTTGTCGG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.0	58.2	830-879 bp	0.666667	74.7
ycf3-trnS-GGA_p3	GCGTAGTGCTTTTTCCCCTA	ATCCCCACATCAGGAATTGC	50.0	50.0	57.9	57.9	1604-1696 bp	0.666667	74.2
ycf3-trnS-GGA_p4	TAGGCGTAGTGCTTTTTCCC	ATCCCCACATCAGGAATTGC	50.0	50.0	57.9	57.9	1607-1699 bp	0.666667	74.2
ycf3-trnS-GGA_p5	TTTTCCCCTGAAGTTGTCGG	CAGTTCCAATGCTAGGCCTT	50.0	50.0	58.0	57.9	832 bp	0.333333	52.1