Hotspot

trnE-UUC-trnT-GGU

LSC · 29305-30055 · 751 bp

Back to Burmannia

Pi 0.0978

MarkerSeek score 82.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 94
pi: 0.0978
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	855-893 bp	1.0	90.6
trnE-UUC-trnT-GGU_p2	GTGCTCTGACCGATTGAACT	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1469-1478 bp	0.666667	75.8
trnE-UUC-trnT-GGU_p3	GTGCTCTGACCGATTGAACT	ATTGTCGCCAATGTGGAAGT	50.0	45.0	57.9	58.1	1893 bp	0.333333	51.9
trnE-UUC-trnT-GGU_p4	GTGCTCTGACCGATTGAACT	TGTCGCCAATGTGGAAGTAG	50.0	50.0	57.9	57.9	1891 bp	0.333333	51.9
trnE-UUC-trnT-GGU_p5	GTGCTCTGACCGATTGAACT	GTCGCCAATGTGGAAGTAGT	50.0	50.0	57.9	57.9	1890 bp	0.333333	51.9