Hotspot

trnS-GCU-trnG-UCC

LSC · 6268-6931 · 664 bp

Back to Burmannia

Pi 0.126
MarkerSeek score 80.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
664
variable_sites
101
pi
0.126
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 TCCACTCAGCCATCTCTCC TACCACTAAACTATACCCGC 57.9 45.0 58.1 54.0 548-883 bp 1.0 52.0
trnS-GCU-trnG-UCC_p2 TCCACTCAGCCATCTCTCC TTACCACTAAACTATACCCGC 57.9 42.9 58.1 54.9 549-884 bp 1.0 52.0
trnS-GCU-trnG-UCC_p3 TCCACTCAGCCATCTCTCC TTTACCACTAAACTATACCCGC 57.9 40.9 58.1 55.6 550-885 bp 1.0 52.0
trnS-GCU-trnG-UCC_p4 TCCACTCAGCCATCTCTCC CTTTTACCACTAAACTATACCCGC 57.9 41.7 58.1 57.4 552-887 bp 1.0 52.0
trnS-GCU-trnG-UCC_p5 TCCACTCAGCCATCTCTCC TTTTACCACTAAACTATACCCGC 57.9 39.1 58.1 56.4 551-886 bp 1.0 52.0