Hotspot

rpl32-trnL-UAG

SSC · 115680-116416 · 737 bp

Back to Bupleurum

Pi 0.0491
MarkerSeek score 86.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
737
variable_sites
209
pi
0.0491
unique_haplotype_count
41
primer_available
yes
alignment_reliability
0.92
markerseek_score
86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCGTTGTCATTGGGGAAAT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 764-960 bp 1.0 91.3
rpl32-trnL-UAG_p2 AGCGTTGTCATTGGGGAAAT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 765-961 bp 1.0 91.3
rpl32-trnL-UAG_p3 AGCGTTGTCATTGGGGAAAT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 774-970 bp 1.0 90.4
rpl32-trnL-UAG_p4 AGCGTTGTCATTGGGGAAAT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.1 57.8 773-969 bp 1.0 89.8
rpl32-trnL-UAG_p5 AGCGTTGTCATTGGGGAAAT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 772-968 bp 1.0 89.8