Hotspot

ndhF-rpl32

SSC · 114282-115514 · 1233 bp

Back to Bupleurum

Pi 0.0324
MarkerSeek score 78.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1233
variable_sites
266
pi
0.0324
unique_haplotype_count
42
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCACGCATATTGATATGTCTGT CCCTTCCCCTTCCAAATATTTT 39.1 40.9 57.8 56.3 1214-1384 bp 1.0 52.0
ndhF-rpl32_p2 TCCACGCATATTGATATGTCTGT CCCTTCCCCTTCCAAATATT 39.1 45.0 57.8 54.6 1214-1384 bp 0.953488 50.1
ndhF-rpl32_p3 TCCACGCATATTGATATGTCTGT CCCTTCCCCTTCCAAATATTT 39.1 42.9 57.8 55.5 1214-1384 bp 0.953488 50.1
ndhF-rpl32_p4 CCACGCATATTGATATGTCTGT CCCTTCCCCTTCCAAATATT 40.9 45.0 56.4 54.6 1213-1383 bp 0.953488 50.1
ndhF-rpl32_p5 CCACGCATATTGATATGTCTGT CCCTTCCCCTTCCAAATATTT 40.9 42.9 56.4 55.5 1213-1383 bp 0.953488 50.1