Hotspot

trnP-UGG-psaJ

LSC · 68158-68588 · 431 bp

Back to Bupleurum

Pi 0.0282

MarkerSeek score 71.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 70
pi: 0.0282
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CGTTACTGCTCAACGGAGAA	TGTCAACGCATCTGGGAAAA	50.0	45.0	57.9	58.0	613-682 bp	1.0	91.3
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	TGTCAACGCATCTGGGAAAA	45.0	45.0	57.9	58.0	546-615 bp	1.0	90.9
trnP-UGG-psaJ_p3	CGTTACTGCTCAACGGAGAA	GGTGCCACGGAAAGATAAGT	50.0	50.0	57.9	57.9	534-603 bp	1.0	90.6
trnP-UGG-psaJ_p4	CAAAACAAACGCGCTACCAA	TGTCAACGCATCTGGGAAAA	45.0	45.0	58.2	58.0	547-616 bp	1.0	90.4
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	TGTCAACGCATCTGGGAAAA	45.0	45.0	57.8	58.0	554-623 bp	1.0	90.3