Hotspot

petA-psbJ

LSC · 64645-65658 · 1014 bp

Back to Bupleurum

Pi 0.0299

MarkerSeek score 86.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 186
pi: 0.0299
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTACGTGTCCAAGGCCTTT	GCAGGTATTCTTGTGATCGGT	50.0	47.6	58.1	58.1	1075-1251 bp	1.0	82.4
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GCAGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	1062-1238 bp	1.0	82.0
petA-psbJ_p3	CTTACGTGTCCAAGGCCTTT	AGCAGGTATTCTTGTGATCGG	50.0	47.6	58.1	57.8	1076-1252 bp	1.0	81.7
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	AGCAGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	1063-1239 bp	1.0	81.3
petA-psbJ_p5	GCATCTGTTATTTTGGCGCA	GCAGGTATTCTTGTGATCGGT	45.0	47.6	57.7	58.1	1044-1220 bp	1.0	81.0