Hotspot

trnT-GGU-psbD

LSC · 33013-34216 · 1204 bp

Back to Bupleurum

Pi 0.018

MarkerSeek score 73.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1204
variable_sites: 161
pi: 0.018
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1343-1446 bp	0.976744	88.3
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1306-1409 bp	1.0	87.8
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1342-1445 bp	0.976744	87.1
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	TCCCTCCGTAACCAGTCATC	50.0	55.0	57.9	58.5	1328-1431 bp	0.976744	86.1
trnT-GGU-psbD_p5	TTGGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.7	58.2	1348-1408 bp	0.930233	82.8