Hotspot

petN-psbM

LSC · 29924-31017 · 1094 bp

Back to Bupleurum

Pi 0.0273

MarkerSeek score 81.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 178
pi: 0.0273
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.0	57.6	1154-1310 bp	1.0	80.5
petN-psbM_p2	AGTAAATCTCGCTTGGGCTG	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	58.0	57.2	1133-1289 bp	1.0	77.5
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1094-1250 bp	0.976744	77.0
petN-psbM_p4	AGTAAATCTCGCTTGGGCTG	TGCTACTGCACTGTTCATTC	50.0	45.0	58.0	56.1	1154-1310 bp	1.0	76.3
petN-psbM_p5	AGTAAATCTCGCTTGGGCTG	AGTTCCTACTGCTTTTCTGCTT	50.0	40.9	58.0	57.9	1133-1289 bp	1.0	75.0