Hotspot

atpH-atpI

LSC · 14596-15391 · 796 bp

Back to Bupleurum

Pi 0.0255

MarkerSeek score 78.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 131
pi: 0.0255
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	918-1012 bp	1.0	87.0
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	878-972 bp	1.0	86.7
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	870-964 bp	1.0	86.5
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.2	58.5	922-1016 bp	1.0	86.3
atpH-atpI_p5	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	860-954 bp	0.953488	85.2