Hotspot

trnS-GCU-trnG-UCC

LSC · 9185-9904 · 720 bp

Back to Bupleurum

Pi 0.0217

MarkerSeek score 75.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 107
pi: 0.0217
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGATCCCT	45.0	40.9	58.0	57.7	670-894 bp	0.930233	69.5
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGATCCCT	50.0	40.9	58.2	57.7	719-943 bp	0.930233	68.3
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAAAGATCCCT	50.0	40.9	57.6	57.7	656-880 bp	0.930233	66.7
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	TGAATCAAACCGAAAGATCCCT	55.0	40.9	58.5	57.7	651-875 bp	0.930233	65.9
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGATCCC	45.0	42.9	58.0	56.2	670-894 bp	0.930233	65.8