Hotspot

rps16-trnQ-UUG

LSC · 6482-7789 · 1308 bp

Back to Bupleurum

Pi 0.0286

MarkerSeek score 74.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1308
variable_sites: 231
pi: 0.0286
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	844-1859 bp	1.0	86.6
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TCGGAGGTTCGAATCCTTTC	45.0	50.0	58.1	57.3	826-1841 bp	1.0	85.5
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	853-1868 bp	1.0	85.4
rps16-trnQ-UUG_p4	CGGATCGTGTCCTTCAAGTC	GGGTTTTGGTCCCGGTATTC	55.0	55.0	58.4	58.5	859-1874 bp	1.0	84.8
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GGGTTTTGGTCCCGGTATTC	55.0	55.0	58.4	58.5	858-1873 bp	1.0	84.8