Hotspot

trnH-GUG-psbA

LSC · 181-528 · 348 bp

Back to Bupleurum

Pi 0.0436

MarkerSeek score 77.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 99
pi: 0.0436
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	ACCGCGCTAATCTTGGTATG	55.0	50.0	58.1	58.1	438-502 bp	0.976744	89.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	452-516 bp	0.976744	88.9
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	451-515 bp	0.976744	88.9
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	453-517 bp	0.976744	88.9
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CGCGCTAATCTTGGTATGGA	55.0	50.0	58.1	57.9	436-500 bp	0.976744	88.8