Hotspot

ycf1

SSC · 123230-128518 · 5289 bp

Back to Bunias

Pi 0.0218
MarkerSeek score 56.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5289
variable_sites
115
pi
0.0218
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AAACCACTACACGTTTTGCT ACGGATAGCAATGATCAAAATCT 40.0 34.8 56.1 56.4 181-184 bp 1.0 45.3
ycf1_p2 AACCACTACACGTTTTGCTT ACGGATAGCAATGATCAAAATCT 40.0 34.8 56.1 56.4 180-183 bp 1.0 45.3
ycf1_p3 ACCACTACACGTTTTGCTTT ACGGATAGCAATGATCAAAATCT 40.0 34.8 56.1 56.4 179-182 bp 1.0 45.3
ycf1_p4 ACCACTACACGTTTTGCTTTTC ACGGATAGCAATGATCAAAATCT 40.9 34.8 58.0 56.4 179-182 bp 1.0 45.3
ycf1_p5 ACCACTACACGTTTTGCTTTT ACGGATAGCAATGATCAAAATCT 38.1 34.8 56.9 56.4 179-182 bp 1.0 45.3