Hotspot

rps15-ycf1

SSC · 122913-123229 · 317 bp

Back to Bunias

Pi 0.0413
MarkerSeek score 72.40
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
317
variable_sites
13
pi
0.0413
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAACAGATCCCCTGCTTTC GCTTGTATGAATCGCTATTGGT 50.0 40.9 57.5 56.9 466-473 bp 1.0 53.9
rps15-ycf1_p2 CAACAGATCCCCTGCTTTCT GCTTGTATGAATCGCTATTGGT 50.0 40.9 57.5 56.9 465-472 bp 1.0 53.9
rps15-ycf1_p3 TCAACAGATCCCCTGCTTTC AGCTTGTATGAATCGCTATTGGT 50.0 39.1 57.5 58.3 467-474 bp 1.0 52.2
rps15-ycf1_p4 CAACAGATCCCCTGCTTTCT AGCTTGTATGAATCGCTATTGGT 50.0 39.1 57.5 58.3 466-473 bp 1.0 52.2
rps15-ycf1_p5 TCAACAGATCCCCTGCTTTC AGCTTGTATGAATCGCTATTGG 50.0 40.9 57.5 56.7 467-474 bp 1.0 51.8