Hotspot

rpl32-trnL

SSC · 112982-113680 · 699 bp

Back to Bunias

Pi 0.0378
MarkerSeek score 73.70
Primer available yes
Label rpl32-trnL

Feature Metadata

label_name
rpl32-trnL
feature_type
igs
region
SSC
length_bp
699
variable_sites
26
pi
0.0378
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_p1 TGGACATCGTTGAAAGCTTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 55.9 57.9 822-831 bp 1.0 65.6
rpl32-trnL_p2 TTGGACATCGTTGAAAGCTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 55.9 57.9 823-832 bp 1.0 65.6
rpl32-trnL_p3 TGGACATCGTTGAAAGCTTT AGCAGCGTGTCTACCAATTT 40.0 45.0 55.9 57.8 815-824 bp 1.0 65.2
rpl32-trnL_p4 TTGGACATCGTTGAAAGCTT AGCAGCGTGTCTACCAATTT 40.0 45.0 55.9 57.8 816-825 bp 1.0 65.2
rpl32-trnL_p5 TGGACATCGTTGAAAGCTTT CTAAGAGCAGCGTGTCTACC 40.0 55.0 55.9 57.8 820-829 bp 1.0 65.0