Hotspot

ndhF-rpl32

SSC · 111994-112822 · 829 bp

Back to Bunias

Pi 0.0411
MarkerSeek score 71.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
829
variable_sites
34
pi
0.0411
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAAAGGGA ACGAATACGCTTTTTCGAGA 47.6 40.0 57.6 55.5 929-941 bp 1.0 51.8
ndhF-rpl32_p2 TGGAAGTGGAATGAAAGGGA ACGAATACGCTTTTTCGAGA 45.0 40.0 56.3 55.5 928-940 bp 1.0 50.0
ndhF-rpl32_p3 CTGGAAGTGGAATGAAAGGGA ACGAATACGCTTTTTCGAGAT 47.6 38.1 57.6 56.1 929-941 bp 1.0 48.3
ndhF-rpl32_p4 CTGGAAGTGGAATGAAAGGG ACGAATACGCTTTTTCGAGA 50.0 40.0 56.0 55.5 929-941 bp 1.0 47.4
ndhF-rpl32_p5 CTGGAAGTGGAATGAAAGGGAT ACGAATACGCTTTTTCGAGA 45.5 40.0 58.0 55.5 929-941 bp 1.0 47.2