Hotspot

rpl16-rpl16

LSC · 80892-82006 · 1115 bp

Back to Bunias

Pi 0.0292
MarkerSeek score 63.70
Primer available yes
Label rpl16-rpl16

Feature Metadata

label_name
rpl16-rpl16
feature_type
igs
region
LSC
length_bp
1115
variable_sites
32
pi
0.0292
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rpl16_p1 GCGGGTCTTACTGTAACTGG GTATGGAGGGAATTGCACGT 55.0 50.0 58.0 58.0 2010-2015 bp 1.0 82.0
rpl16-rpl16_p2 TCGCCCTGCTTCTATTTGTC GTATGGAGGGAATTGCACGT 50.0 50.0 58.0 58.0 1927-1932 bp 1.0 81.9
rpl16-rpl16_p3 CGCCCTGCTTCTATTTGTCT GTATGGAGGGAATTGCACGT 50.0 50.0 58.0 58.0 1926-1931 bp 1.0 81.9
rpl16-rpl16_p4 CCTTTCCCAGAACCCATACG GTATGGAGGGAATTGCACGT 55.0 50.0 58.0 58.0 2037-2042 bp 1.0 81.7
rpl16-rpl16_p5 ACACATCAATTCTCGAGCCC GTATGGAGGGAATTGCACGT 50.0 50.0 58.0 58.0 2371-2374 bp 1.0 81.5