Hotspot

psbG-ndhC

LSC · 49519-49579 · 61 bp

Back to Bunias

Pi 0.0656
MarkerSeek score 76.10
Primer available yes
Label psbG-ndhC

Feature Metadata

label_name
psbG-ndhC
feature_type
igs
region
LSC
length_bp
61
variable_sites
4
pi
0.0656
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbG-ndhC_p1 GGCTGGAAAGTCTTGACCAA TGTACTGGGGGTATCTGCTT 50.0 50.0 58.0 57.7 264 bp 1.0 79.0
psbG-ndhC_p2 GGCTGGAAAGTCTTGACCAA ATGTACTGGGGGTATCTGCT 50.0 50.0 58.0 57.5 265 bp 1.0 77.1
psbG-ndhC_p3 GGCTGGAAAGTCTTGACCAA TGGCGAAAAGGAGCATTAGA 50.0 45.0 58.0 57.2 191 bp 1.0 75.3
psbG-ndhC_p4 GGCTGGAAAGTCTTGACCAA GATGTACTGGGGGTATCTGC 50.0 55.0 58.0 57.2 266 bp 1.0 74.3
psbG-ndhC_p5 GGCTGGAAAGTCTTGACCAA TATGCATGGCGAAAAGGAGC 50.0 50.0 58.0 59.0 197 bp 1.0 73.7