Hotspot

trnE-trnT

LSC · 30255-31068 · 814 bp

Back to Bunias

Pi 0.0298
MarkerSeek score 67.60
Primer available yes
Label trnE-trnT

Feature Metadata

label_name
trnE-trnT
feature_type
igs
region
LSC
length_bp
814
variable_sites
24
pi
0.0298
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-trnT_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 952-955 bp 1.0 81.4
trnE-trnT_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 931-934 bp 1.0 81.0
trnE-trnT_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 928-931 bp 1.0 80.9
trnE-trnT_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 914-917 bp 1.0 80.7
trnE-trnT_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 893-896 bp 1.0 80.7