Hotspot

psbM-trnD

LSC · 28514-29521 · 1008 bp

Back to Bunias

Pi 0.0238
MarkerSeek score 61.40
Primer available yes
Label psbM-trnD

Feature Metadata

label_name
psbM-trnD
feature_type
igs
region
LSC
length_bp
1008
variable_sites
24
pi
0.0238
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD_p1 TGAGAATGAAGAGTGCAGTAGC GTTCGAGCCCCGTCAGTC 45.5 66.7 58.1 60.1 1078-1090 bp 1.0 41.8
psbM-trnD_p2 GAGAATGAAGAGTGCAGTAGC GTTCGAGCCCCGTCAGTC 47.6 66.7 56.4 60.1 1077-1089 bp 1.0 41.8
psbM-trnD_p3 AGGAATGAGAATGAAGAGTGCA GTTCGAGCCCCGTCAGTC 40.9 66.7 57.4 60.1 1083-1095 bp 1.0 41.8
psbM-trnD_p4 TGAGAATGAAGAGTGCAGTAGC GAGCACCGCCCTGTCAAG 45.5 66.7 58.1 60.7 1109-1121 bp 1.0 41.7
psbM-trnD_p5 TGAGAATGAAGAGTGCAGTAGC GCCCTGTCAAGGCGGAAG 45.5 66.7 58.1 60.7 1102-1114 bp 1.0 41.7