Hotspot

atpA-atpF

LSC · 11255-11320 · 66 bp

Back to Bunias

Pi 0.0462
MarkerSeek score 76.60
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
66
variable_sites
3
pi
0.0462
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 GCCCACTTGAAGTACGGTAC ACAAGGAGCTATAGGAACCCT 55.0 47.6 58.3 57.6 282-283 bp 1.0 67.5
atpA-atpF_p2 CCCACTTGAAGTACGGTACC ACAAGGAGCTATAGGAACCCT 55.0 47.6 57.6 57.6 281-282 bp 1.0 66.8
atpA-atpF_p3 CCATCGCCCACTTGAAGTAC ACAAGGAGCTATAGGAACCCT 55.0 47.6 58.6 57.6 287-288 bp 1.0 64.7
atpA-atpF_p4 GCCCACTTGAAGTACGGTAC ACAAGGAGCTATAGGAACCC 55.0 50.0 58.3 56.0 282-283 bp 1.0 62.9
atpA-atpF_p5 CCCACTTGAAGTACGGTACC ACAAGGAGCTATAGGAACCC 55.0 50.0 57.6 56.0 281-282 bp 1.0 62.1