Hotspot

trnR-atpA

LSC · 9455-9730 · 276 bp

Back to Bunias

Pi 0.0435
MarkerSeek score 71.80
Primer available yes
Label trnR-atpA

Feature Metadata

label_name
trnR-atpA
feature_type
igs
region
LSC
length_bp
276
variable_sites
12
pi
0.0435
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-atpA_p1 GCGAAAAGCGTCCATTGTC ACATTAACCGCTGAAGCAGA 52.6 45.0 58.0 57.5 445-458 bp 1.0 70.3
trnR-atpA_p2 GCGAAAAGCGTCCATTGTC AGACATTAACCGCTGAAGCA 52.6 45.0 58.0 57.5 447-460 bp 1.0 70.3
trnR-atpA_p3 GCGAAAAGCGTCCATTGTC GACATTAACCGCTGAAGCAG 52.6 50.0 58.0 57.2 446-459 bp 1.0 67.6
trnR-atpA_p4 GCGAAAAGCGTCCATTGTC TAACCGCTGAAGCAGAAAGC 52.6 50.0 58.0 58.8 441-454 bp 1.0 67.4
trnR-atpA_p5 GCGAAAAGCGTCCATTGTC ACCAAGACATTAACCGCTGA 52.6 45.0 58.0 57.1 451-464 bp 1.0 67.3