Hotspot

ycf1

SSC · 119798-125284 · 5487 bp

Back to Bulbophyllum

Pi 0.0283

MarkerSeek score 68.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5487
variable_sites: 784
pi: 0.0283
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTGTTCTCTGCCCATCCAA	CCGCCCAGTTTATCGACTTT	50.0	50.0	57.8	58.3	1000-1030 bp	0.947368	84.7
ycf1_p2	CTTGTTCTCTGCCCATCCAA	GATTCCGTAGAACTCCACCG	50.0	55.0	57.8	57.8	607-640 bp	0.894737	83.6
ycf1_p3	AACCTCTCCCTCTTCCGTTT	CCGCCCAGTTTATCGACTTT	50.0	50.0	58.3	58.3	783-810 bp	0.526316	63.3
ycf1_p4	AACCTCTCCCTCTTCCGTTT	GATTCCGTAGAACTCCACCG	50.0	55.0	58.3	57.8	390-420 bp	0.473684	62.4
ycf1_p5	AAACCTCTCCCTCTTCCGTT	GATTCCGTAGAACTCCACCG	50.0	55.0	58.3	57.8	391-421 bp	0.473684	62.3