Hotspot

rpl32-trnL-UAG

SSC · 112073-112612 · 540 bp

Back to Bulbophyllum

Pi 0.0439
MarkerSeek score 80.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
540
variable_sites
116
pi
0.0439
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAGCTACTGAGGAACAACGG ATCCGCATAAAAGAGCTGCA 55.0 45.0 58.0 58.0 1894-2841 bp 0.315789 58.7
rpl32-trnL-UAG_p2 GAGCTACTGAGGAACAACGG TCCGCATAAAAGAGCTGCAT 55.0 45.0 58.0 58.0 1893-2840 bp 0.315789 58.7
rpl32-trnL-UAG_p3 GCTTCCTTCGTAACTCCAGG ATCCGCATAAAAGAGCTGCA 55.0 45.0 58.0 58.0 1814-2761 bp 0.315789 58.7
rpl32-trnL-UAG_p4 GCTTCCTTCGTAACTCCAGG TCCGCATAAAAGAGCTGCAT 55.0 45.0 58.0 58.0 1813-2760 bp 0.315789 58.7
rpl32-trnL-UAG_p5 GAGCTACTGAGGAACAACGG TGGCGAAACCTTGGGTAAAA 55.0 45.0 58.0 57.9 1774-2721 bp 0.315789 58.6