Hotspot

rpl16-rps3

LSC · 84746-84924 · 179 bp

Back to Bulbophyllum

Pi 0.0517

MarkerSeek score 80.50

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 39
pi: 0.0517
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TAACGAGTCACACACTGAGC	AGGCATCAAAATTTGGATATTTGT	50.0	29.2	57.6	55.9	232-267 bp	1.0	52.0
rpl16-rps3_p2	TAACGAGTCACACACTGAGC	GGCATCAAAATTTGGATATTTGT	50.0	30.4	57.6	54.5	231-266 bp	1.0	52.0
rpl16-rps3_p3	TAACGAGTCACACACTGAGC	AGGCATCAAAATTTGGATATTTG	50.0	30.4	57.6	54.2	232-267 bp	1.0	52.0
rpl16-rps3_p4	AACGAGTCACACACTGAGC	AGGCATCAAAATTTGGATATTTGT	52.6	29.2	57.7	55.9	231-266 bp	1.0	52.0
rpl16-rps3_p5	ACGAGTCACACACTGAGCAT	AGGCATCAAAATTTGGATATTTGT	50.0	29.2	59.3	55.9	230-265 bp	1.0	52.0