Hotspot

psbB-psbT

LSC · 75583-76072 · 490 bp

Back to Bulbophyllum

Pi 0.0606

MarkerSeek score 80.70

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 131
pi: 0.0606
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TGGAGATCCAACTACAAGGAGA	GGAATGTATAAACCAATGCTTCCA	45.5	37.5	57.9	57.5	430-679 bp	0.947368	49.9
psbB-psbT_p2	TGGAGATCCAACTACAAGGAGA	AGGAATGTATAAACCAATGCTTCC	45.5	37.5	57.9	57.3	431-680 bp	0.947368	49.9
psbB-psbT_p3	GGAGATCCAACTACAAGGAGAC	GGAATGTATAAACCAATGCTTCCA	50.0	37.5	57.6	57.5	429-678 bp	0.947368	49.9
psbB-psbT_p4	TGGAGATCCAACTACAAGGAG	GGAATGTATAAACCAATGCTTCCA	47.6	37.5	56.5	57.5	430-679 bp	0.947368	49.9
psbB-psbT_p5	GGAGATCCAACTACAAGGAGAC	AGGAATGTATAAACCAATGCTTCC	50.0	37.5	57.6	57.3	430-679 bp	0.947368	49.9