Hotspot

psbE-petL

LSC · 65775-66873 · 1099 bp

Back to Bulbophyllum

Pi 0.0277

MarkerSeek score 77.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1099
variable_sites: 163
pi: 0.0277
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTTGAAGCAGCCAGTAAAA	50.0	40.0	57.9	55.5	713-1307 bp	1.0	71.4
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGTTGAAGCAGCCAGTAAAA	50.0	40.0	57.9	55.5	726-1320 bp	1.0	71.1
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGTTGAAGCAGCCAGTAAAA	45.0	40.0	58.2	55.5	722-1316 bp	1.0	70.4
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGTTGAAGCAGCCAGTAAAA	50.0	40.0	57.8	55.5	721-1315 bp	1.0	70.3
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGTTGAAGCAGCCAGTAAAA	50.0	40.0	58.2	55.5	715-1309 bp	1.0	69.9